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MGLL Monoclonal antibody

MGLL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A3E11

Cat no : 67846-1-Ig

Print datasheet

Synonyms

HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, monoglyceride lipase

验证数据展示

  • WB analysis using 67846-1-Ig

    WB analysis using 67846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67846-1-Ig (MGLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

经过测试的应用

Positive WB detected inTHP-1 cells, A549 cells, HeLa cells, human platelets tissue
Planning an IHC experiment? We recommend our IHCeasy MGLL Ready-To-Use IHC Kit. MGLL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67846-1-Ig targets MGLL in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen MGLL fusion protein Ag7052 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name monoglyceride lipase
Synonyms HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, monoglyceride lipase
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
RRIDAB_2918608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

实验方案

Product Specific Protocols
WB protocol for MGLL antibody 67846-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols