• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67868-1-Ig

    WB analysis using 67868-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67868-1-Ig (AATF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 67868-1-Ig

    WB analysis of Neuro-2a using 67868-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67868-1-Ig (AATF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67868-1-Ig

    WB analysis of HepG2 using 67868-1-Ig

    WB result of AATF antibody (67868-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells.

  • FC experiment of HeLa using 67868-1-Ig

    FC experiment of HeLa using 67868-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human AATF (67868-1-Ig, Clone:2A6G6) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

AATF Monoclonal antibody

AATF Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67868-1-Ig

产品说明书

CloneNo. 2A6G6
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA, FC (Intra) and More (1)

别名

Protein AATF, HSPC277, DED, CHE1, CHE 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHCT 116 cells, Neuro-2a cells, HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67868-1-Ig targets AATF in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 AATF fusion protein Ag0195 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apoptosis antagonizing transcription factor
别名 Protein AATF, HSPC277, DED, CHE1, CHE 1
计算分子量 63 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC000591
基因名称 AATF
Gene ID (NCBI) 26574
RRIDAB_2918626
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NY61
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AATF antibody 67868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

AATF is Overexpressed in Human Head and Neck Squamous Cell Carcinoma and Regulates STAT3/Survivin Signaling

Authors - Lin Fu
View Article
humanWB,IHC

Onco Targets Ther

AATF is Overexpressed in Human Bladder Cancer and Regulates Chemo-Sensitivity Through Survivin.

Authors - Shutao Tan
View Article