验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, PC-3 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67869-1-Ig targets AES in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | AES fusion protein Ag31320 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | amino-terminal enhancer of split |
| 别名 | AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5 |
| 计算分子量 | 264 aa, 29 kDa |
| 观测分子量 | 29-31 kDa |
| GenBank蛋白编号 | BC113735 |
| 基因名称 | AES |
| Gene ID (NCBI) | 166 |
| RRID | AB_2918627 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q08117 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AES antibody 67869-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |