验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HT-29 cells, COLO320 cells, SW480 cells, NCCIT cells, BxPC-3 cells, U-87 MG cells, HL-60 cells, Pig colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67877-1-Ig targets PLEKHB2 in WB, ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 | PLEKHB2 fusion protein Ag9231 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family B (evectins) member 2 |
| 别名 | Evectin 2, EVT2, FLJ20783, FLJ23679, FLJ30436, PLEKHB2 |
| 计算分子量 | 222 aa, 25 kDa |
| 观测分子量 | 19-25;30-34 kDa |
| GenBank蛋白编号 | BC013991 |
| 基因名称 | PLEKHB2 |
| Gene ID (NCBI) | 55041 |
| RRID | AB_2918634 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96CS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHB2, also named as EVT2 (Evectin-2), is involved in retrograde transport of recycling endosomes. PLEKHB2 has been identified as post-Golgi proteins of unknown function that have a PH domain that typically binds polyphosphoinositides. PLEKHB2 is expressed in a broad range of tissues. PLEKHB2 has some isoforms with MW 19-26 kDa and 34 kDa. This antibody recognizes all the isoforms of PLEKHB2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHB2 antibody 67877-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |