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WIF1 Monoclonal antibody

WIF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A3

Cat no : 67904-1-Ig

Print datasheet

Synonyms

WIF 1, WIF1, WNT inhibitory factor 1

验证数据展示

  • WB analysis using 67904-1-Ig

    WB analysis using 67904-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67904-1-Ig (WIF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inCaco-2 cells, SW480 cells, COLO 320 cells, HT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67904-1-Ig targets WIF1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen WIF1 fusion protein Ag23972 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WNT inhibitory factor 1
Synonyms WIF 1, WIF1, WNT inhibitory factor 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC018037
Gene Symbol WIF1
Gene ID (NCBI) 11197
RRIDAB_2918660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y5W5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WIF1 antibody 67904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone

Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway

Authors - Yuanxi Leng
View Article
humanWB

Aging (Albany NY)

Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma

Authors - Sen Qin
View Article