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验证数据展示

  • WB analysis using 67921-1-Ig

    WB analysis using 67921-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67921-1-Ig (NUDT22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 67921-1-Ig

    FC experiment of Jurkat using 67921-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NUDT22 (67921-1-Ig, Clone:3C3H9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NUDT22 Monoclonal antibody

NUDT22 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67921-1-Ig

产品说明书

CloneNo. 3C3H9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

WB, ELISA, FC (Intra)

别名

UDPG pyrophosphatase, PP11246, NUDT 22, Nucleoside diphosphate-linked moiety X motif 22, EC:3.6.1.45

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67921-1-Ig targets NUDT22 in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 NUDT22 fusion protein Ag14787 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 22
别名 UDPG pyrophosphatase, PP11246, NUDT 22, Nucleoside diphosphate-linked moiety X motif 22, EC:3.6.1.45
计算分子量 303 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC006129
基因名称 NUDT22
Gene ID (NCBI) 84304
RRIDAB_2918673
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRQ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human NUDT22 belongs to the diverse NUDIX family of proteins, is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate.

实验方案

Product Specific Protocols
WB protocol for NUDT22 antibody 67921-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols