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验证数据展示

  • WB analysis using 67938-1-Ig

    WB analysis using 67938-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67938-1-Ig (PSMA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included

PSMA3 Monoclonal antibody

PSMA3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67938-1-Ig

产品说明书

CloneNo. 1A8C9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67938-1-Ig targets PSMA3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PSMA3 fusion protein Ag30552 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 3
别名 HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3
计算分子量 255 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC029402
基因名称 PSMA3
Gene ID (NCBI) 5684
RRIDAB_2918690
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA3 antibody 67938-1-IgDownload protocol
IHC protocol for PSMA3 antibody 67938-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols