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PSMA3 Monoclonal antibody

PSMA3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A8C9

Cat no : 67938-1-Ig

Print datasheet

Synonyms

HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

验证数据展示

  • WB analysis using 67938-1-Ig

    WB analysis using 67938-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67938-1-Ig (PSMA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included
  • IHC staining of human liver cancer using 67938-1-Ig

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA3 antibody included

经过测试的应用

Positive WB detected inA431 cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA3 Ready-To-Use IHC Kit. PSMA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67938-1-Ig targets PSMA3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PSMA3 fusion protein Ag30552 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 3
Synonyms HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3
Calculated Molecular Weight 255 aa, 28 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC029402
Gene Symbol PSMA3
Gene ID (NCBI) 5684
RRIDAB_2918690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA3 antibody 67938-1-IgDownload protocol
IHC protocol for PSMA3 antibody 67938-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols