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验证数据展示

  • WB analysis using 67940-1-Ig

    WB analysis using 67940-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67940-1-Ig (LARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IF Staining of HeLa using 67940-1-Ig

    IF Staining of HeLa using 67940-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LARS antibody (67940-1-Ig, Clone: 1E8D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

LARS Monoclonal antibody

LARS Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67940-1-Ig

产品说明书

CloneNo. 1E8D4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FLJ10595, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LARS1 Ready-To-Use IHC Kit. LARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67940-1-Ig targets LARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 LARS fusion protein Ag15944 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leucyl-tRNA synthetase
别名 FLJ10595, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS
计算分子量 1176 aa, 134 kDa
观测分子量 135-140 kDa
GenBank蛋白编号BC151214
基因名称 LARS
Gene ID (NCBI) 51520
RRIDAB_2918692
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P2J5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LARS antibody 67940-1-IgDownload protocol
IF protocol for LARS antibody 67940-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols