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验证数据展示

  • WB analysis using 67941-1-Ig

    WB analysis using 67941-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

PUF-A,KIAA0020 Monoclonal antibody

PUF-A,KIAA0020 Monoclonal Antibody for WB, ELISA
Cat No. 67941-1-Ig

产品说明书

CloneNo. 1G4B7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67941-1-Ig targets PUF-A,KIAA0020 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PUF-A,KIAA0020 fusion protein Ag31324 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0020
别名 HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5
计算分子量 648 aa, 74 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC016137
基因名称 PUF-A/KIAA0020
Gene ID (NCBI) 9933
RRIDAB_2918693
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PUF-A,KIAA0020 antibody 67941-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols