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验证数据展示

  • WB analysis using 68003-1-Ig

    WB analysis using 68003-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of LNCaP using 68003-1-Ig

    WB analysis of LNCaP using 68003-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68003-1-Ig

    IF Staining of HeLa using 68003-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCAS2 antibody (68003-1-Ig, Clone: 2A12G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

BCAS2 Monoclonal antibody

BCAS2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68003-1-Ig

产品说明书

CloneNo. 2A12G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68003-1-Ig targets BCAS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BCAS2 fusion protein Ag21309 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 breast carcinoma amplified sequence 2
别名 Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2
计算分子量 26 kDa
观测分子量28 kDa
GenBank蛋白编号BC005285
基因名称 BCAS2
Gene ID (NCBI) 10286
RRIDAB_2918750
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75934
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.

实验方案

Product Specific Protocols
WB protocol for BCAS2 antibody 68003-1-IgDownload protocol
IF protocol for BCAS2 antibody 68003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols