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BCAS2 Monoclonal antibody

BCAS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12G1

Cat no : 68003-1-Ig

Print datasheet

Synonyms

BCAS2, DAM1, Pre mRNA splicing factor SPF27

验证数据展示

  • WB analysis using 68003-1-Ig

    WB analysis using 68003-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of LNCaP using 68003-1-Ig

    WB analysis of LNCaP using 68003-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68003-1-Ig targets BCAS2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen BCAS2 fusion protein Ag21309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name breast carcinoma amplified sequence 2
Synonyms BCAS2, DAM1, Pre mRNA splicing factor SPF27
Calculated Molecular Weight 26 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC005285
Gene Symbol BCAS2
Gene ID (NCBI) 10286
RRIDAB_2918750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75934
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.

实验方案

Product Specific Protocols
WB protocol for BCAS2 antibody 68003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols