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SFXN1 Monoclonal antibody

SFXN1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D9F1

Cat no : 68024-1-Ig

Print datasheet

Synonyms

FLJ12876, SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

验证数据展示

  • WB analysis using 68024-1-Ig

    WB analysis using 68024-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of MCF-7 using 68024-1-Ig

    IF Staining of MCF-7 using 68024-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, Rat brain tissue, Mouse brain tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68024-1-Ig targets SFXN1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen SFXN1 fusion protein Ag2985 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sideroflexin 1
Synonyms FLJ12876, SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein
Calculated Molecular Weight35.6 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC020517
Gene Symbol SFXN1
Gene ID (NCBI) 94081
RRIDAB_2918768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H9B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

实验方案

Product Specific Protocols
WB protocol for SFXN1 antibody 68024-1-IgDownload protocol
IF protocol for SFXN1 antibody 68024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols