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验证数据展示

  • WB analysis using 68025-1-Ig

    WB analysis using 68025-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

SLC39A3 Monoclonal antibody

SLC39A3 Monoclonal Antibody for WB, ELISA
Cat No. 68025-1-Ig

产品说明书

CloneNo. 1A11F6

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled HepG2 cells, HepG2 cells, 37°C incubated A549 cells, LNCaP cells, Jurkat cells, K-562 cells, unboiled A549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68025-1-Ig targets SLC39A3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 SLC39A3 fusion protein Ag14289 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 39 (zinc transporter), member 3
别名 SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3
计算分子量 314 aa, 34 kDa
观测分子量32 kDa
GenBank蛋白编号BC005869
基因名称 SLC39A3
Gene ID (NCBI) 29985
RRIDAB_2918769
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRY0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC39A3 antibody 68025-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols