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验证数据展示

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68037-1-Ig

产品说明书

CloneNo. 1G12C6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

别名

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B, Actin-related protein 3B, Actin-related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP3-beta, ARP4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMOLT-4 cells, pig brain tissue, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68037-1-Ig targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 ACTR3B fusion protein Ag10218 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog B (yeast)
别名 1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B, Actin-related protein 3B, Actin-related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP3-beta, ARP4
计算分子量 418 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC008682
基因名称 ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2918779
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P1U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 68037-1-IgDownload protocol
IHC protocol for ACTR3B antibody 68037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols