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Conjugate
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SMS Monoclonal antibody

SMS Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G8E9

Cat no : 68040-1-Ig

Print datasheet

Synonyms

Spermine synthase, SpS, SPMSY, MRSR, EC:2.5.1.22

验证数据展示

  • WB analysis using 68040-1-Ig

    WB analysis using 68040-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68040-1-Ig

    IF Staining of HeLa using 68040-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 68040-1-Ig

    FC experiment of HeLa using 68040-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, HCT 116 cells, HEK-293 cells, Hela cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68040-1-Ig targets SMS in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SMS fusion protein Ag8840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name spermine synthase
Synonyms Spermine synthase, SpS, SPMSY, MRSR, EC:2.5.1.22
Calculated Molecular Weight 366 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC009898
Gene Symbol SMS
Gene ID (NCBI) 6611
RRIDAB_2918782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMS antibody 68040-1-IgDownload protocol
IF protocol for SMS antibody 68040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols