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TPD52L2 Monoclonal antibody

TPD52L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B3C6

Cat no : 68077-1-Ig

Print datasheet

Synonyms

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

验证数据展示

  • WB analysis using 68077-1-Ig

    WB analysis using 68077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 68077-1-Ig

    IHC staining of human breast cancer using 68077-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPD52L2 antibody included
  • IF Staining of HCT 116 using 68077-1-Ig

    IF Staining of HCT 116 using 68077-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using TPD52L2 antibody (68077-1-Ig, Clone: 3B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inCalu-3 cells, NCI-H1299 cells, MCF-7 cells, C6 cells, Neuro-2a cells, HCT 116 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy TPD52L2 Ready-To-Use IHC Kit. TPD52L2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68077-1-Ig targets TPD52L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tumor protein D52-like 2
Synonyms D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC006804
Gene Symbol TPD52L2
Gene ID (NCBI) 7165
RRIDAB_2918815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43399
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

实验方案

Product Specific Protocols
WB protocol for TPD52L2 antibody 68077-1-IgDownload protocol
IHC protocol for TPD52L2 antibody 68077-1-IgDownload protocol
IF protocol for TPD52L2 antibody 68077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols