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IL-11 Monoclonal antibody

IL-11 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3A12

Cat no : 68135-1-Ig

Print datasheet

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

验证数据展示

  • WB analysis using 68135-1-Ig

    WB analysis using 68135-1-Ig

    Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSaos-2 supernatant cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68135-1-Ig targets IL-11 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IL-11 fusion protein Ag11199 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
Full Name interleukin 11
Synonyms IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC012506
Gene Symbol IL-11
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.

实验方案

Product Specific Protocols
WB protocol for IL-11 antibody 68135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols