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验证数据展示

  • WB analysis using 68140-1-Ig

    WB analysis using 68140-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68140-1-Ig (ACOT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

ACOT7 Monoclonal antibody

ACOT7 Monoclonal Antibody for WB, ELISA
Cat No. 68140-1-Ig

产品说明书

CloneNo. 1E9C7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Rat, Mouse, Rabbit, Pig

应用

WB, ELISA

别名

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68140-1-Ig targets ACOT7 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, Mouse, Rabbit, Pig
免疫原 ACOT7 fusion protein Ag8975 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 7
别名 ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1
计算分子量 370 aa, 40 kDa
观测分子量37 kDa
GenBank蛋白编号BC017365
基因名称 ACOT7
Gene ID (NCBI) 11332
RRIDAB_2923667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00154
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824).

实验方案

Product Specific Protocols
WB protocol for ACOT7 antibody 68140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols