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验证数据展示

  • WB analysis using 68158-1-Ig

    WB analysis using 68158-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 68158-1-Ig

    WB analysis of U2OS using 68158-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 68158-1-Ig

    IF Staining of C2C12 using 68158-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TPM3 antibody (68158-1-Ig, Clone: 1F10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

TPM3 Monoclonal antibody

TPM3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68158-1-Ig

产品说明书

CloneNo. 1F10B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig heart tissue, U2OS cells, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue
Positive IF/ICC detected inC2C12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68158-1-Ig targets TPM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 TPM3 fusion protein Ag28568 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tropomyosin 3
别名 Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin
计算分子量 33 kDa
观测分子量33-37 kDa
GenBank蛋白编号BC008407
基因名称 TPM3
Gene ID (NCBI) 7170
RRIDAB_3085044
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPM3 antibody 68158-1-IgDownload protocol
IF protocol for TPM3 antibody 68158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols