• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TPM3 Monoclonal antibody

TPM3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10B3

Cat no : 68158-1-Ig

Print datasheet

Synonyms

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

验证数据展示

  • WB analysis using 68158-1-Ig

    WB analysis using 68158-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 68158-1-Ig

    WB analysis of U2OS using 68158-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 68158-1-Ig

    IF Staining of C2C12 using 68158-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TPM3 antibody (68158-1-Ig, Clone: 1F10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inpig heart tissue, U2OS cells, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue
Positive IF/ICC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68158-1-Ig targets TPM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen TPM3 fusion protein Ag28568 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tropomyosin 3
Synonyms Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33-37 kDa
GenBank Accession NumberBC008407
Gene Symbol TPM3
Gene ID (NCBI) 7170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPM3 antibody 68158-1-IgDownload protocol
IF protocol for TPM3 antibody 68158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols