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LANCL1 Monoclonal antibody

LANCL1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat , pig

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2H6E8

Cat no : 68160-1-Ig

Print datasheet

Synonyms

GPR69A, LanC like protein 1, LANCL1, p40

验证数据展示

  • WB analysis using 68160-1-Ig

    WB analysis using 68160-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of mouse brain using 68160-1-Ig

    IP experiment of mouse brain using 68160-1-Ig

    IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug.

  • IF Staining of U-251 using 68160-1-Ig

    IF Staining of U-251 using 68160-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68160-1-Ig targets LANCL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat , pig
Immunogen LANCL1 fusion protein Ag32665 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name LanC lantibiotic synthetase component C-like 1 (bacterial)
Synonyms GPR69A, LanC like protein 1, LANCL1, p40
Calculated Molecular Weight 399 aa, 45 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC028685
Gene Symbol LANCL1
Gene ID (NCBI) 10314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

实验方案

Product Specific Protocols
WB protocol for LANCL1 antibody 68160-1-IgDownload protocol
IF protocol for LANCL1 antibody 68160-1-IgDownload protocol
IP protocol for LANCL1 antibody 68160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols