• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68160-1-Ig

    WB analysis using 68160-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of mouse brain using 68160-1-Ig

    IP experiment of mouse brain using 68160-1-Ig

    IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug.

  • IF Staining of U-251 using 68160-1-Ig

    IF Staining of U-251 using 68160-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

LANCL1 Monoclonal antibody

LANCL1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68160-1-Ig

产品说明书

CloneNo. 2H6E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat , pig

应用

WB, IF/ICC, IP, ELISA

别名

GPR69A, LanC like protein 1, LANCL1, p40

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68160-1-Ig targets LANCL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat , pig
免疫原 LANCL1 fusion protein Ag32665 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LanC lantibiotic synthetase component C-like 1 (bacterial)
别名 GPR69A, LanC like protein 1, LANCL1, p40
计算分子量 399 aa, 45 kDa
观测分子量40 kDa
GenBank蛋白编号BC028685
基因名称 LANCL1
Gene ID (NCBI) 10314
RRIDAB_2923679
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

实验方案

Product Specific Protocols
WB protocol for LANCL1 antibody 68160-1-IgDownload protocol
IF protocol for LANCL1 antibody 68160-1-IgDownload protocol
IP protocol for LANCL1 antibody 68160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols