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MRPP3 Monoclonal antibody

MRPP3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9A10

Cat no : 68168-1-Ig

Print datasheet

Synonyms

KIAA0391, MRPP3

验证数据展示

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (MRPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68168-1-Ig

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells, pig liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68168-1-Ig targets MRPP3 in WB, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen MRPP3 fusion protein Ag15629 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA0391
Synonyms KIAA0391, MRPP3
Calculated Molecular Weight 583 aa, 67 kDa
Observed Molecular Weight 65-67 kDa
GenBank Accession NumberBC032221
Gene Symbol MRPP3
Gene ID (NCBI) 9692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15091
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

实验方案

Product Specific Protocols
WB protocol for MRPP3 antibody 68168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols