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ARL8A/ARL8B Monoclonal antibody

ARL8A/ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4C3

Cat no : 68186-1-Ig

Print datasheet

Synonyms

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

验证数据展示

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of C6 using 68186-1-Ig

    IF Staining of C6 using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of Neuro-2a using 68186-1-Ig

    IF Staining of Neuro-2a using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A549 using 68186-1-Ig

    IF Staining of A549 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • IF Staining of HEK-293 using 68186-1-Ig

    IF Staining of HEK-293 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrabbit brain tissue, LNCaP cells, human placenta tissue, HeLa cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA549 cells, C6 cells, Neuro-2a cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68186-1-Ig targets ARL8A/ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen ARL8A/ARL8B fusion protein Ag10758 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 8A
Synonyms ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight18 kDa, 21 kDa
GenBank Accession NumberBC015408
Gene Symbol ARL8A
Gene ID (NCBI) 127829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96BM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.

实验方案

Product Specific Protocols
WB protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
IF protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols