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验证数据展示

  • WB analysis using 68187-1-Ig

    WB analysis using 68187-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 68187-1-Ig

    IHC staining of human colon cancer using 68187-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PABPC4 antibody included
  • IHC staining of human colon cancer using 68187-1-Ig

    IHC staining of human colon cancer using 68187-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PABPC4 antibody included
  • IF Staining of A431 using 68187-1-Ig

    IF Staining of A431 using 68187-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PABPC4 antibody (68187-1-Ig, Clone: 1E6B3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PABPC4 Monoclonal antibody

PABPC4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68187-1-Ig

产品说明书

CloneNo. 1E6B3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

Activated platelet protein 1, APP 1, APP1, FLJ43938, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy PABPC4 Ready-To-Use IHC Kit. PABPC4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68187-1-Ig targets PABPC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PABPC4 fusion protein Ag6813 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 poly(A) binding protein, cytoplasmic 4 (inducible form)
别名 Activated platelet protein 1, APP 1, APP1, FLJ43938, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4
计算分子量 70 kDa
观测分子量71 kDa
GenBank蛋白编号BC065540
基因名称 PABPC4
Gene ID (NCBI) 8761
RRIDAB_2935277
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13310
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Poly(A)-binding protein (PABP), a nucleocytoplasmic shuttling protein, has a fundamental role in promoting gene expression by enhancing mRNA translation and stability(PMID: 21940797). Poly(A)-binding protein 4 (PABPC4), one of the homologs of PABP, was initially identified as a human T-cell activation-induced protein. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules(NCBI).

实验方案

Product Specific Protocols
WB protocol for PABPC4 antibody 68187-1-IgDownload protocol
IHC protocol for PABPC4 antibody 68187-1-IgDownload protocol
IF protocol for PABPC4 antibody 68187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols