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CAPZB Monoclonal antibody

CAPZB Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F9G2

Cat no : 68190-1-Ig

Print datasheet

Synonyms

F-actin-capping protein subunit beta, CapZbeta, CapZ beta, 1F9G2

验证数据展示

  • WB analysis using 68190-1-Ig

    WB analysis using 68190-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68190-1-Ig (CAPZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of mouse testis using 68190-1-Ig

    IHC staining of mouse testis using 68190-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68190-1-Ig (CAPZB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPZB antibody included
  • FC experiment of Jurkat using 68190-1-Ig

    FC experiment of Jurkat using 68190-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CAPZB (68190-1-Ig, Clone:1F9G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 68190-1-Ig

    IF Staining of A431 using 68190-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CAPZB antibody (68190-1-Ig, Clone: 1F9G2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CAPZB Ready-To-Use IHC Kit. CAPZB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68190-1-Ig targets CAPZB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CAPZB fusion protein Ag19373 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament) muscle Z-line, beta
Synonyms F-actin-capping protein subunit beta, CapZbeta, CapZ beta, 1F9G2
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC109241
Gene Symbol CAPZB
Gene ID (NCBI) 832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPZB antibody 68190-1-IgDownload protocol
IHC protocol for CAPZB antibody 68190-1-IgDownload protocol
IF protocol for CAPZB antibody 68190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols