• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68208-1-Ig

    WB analysis using 68208-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of rat heart using 68208-1-Ig

    WB analysis of rat heart using 68208-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 68208-1-Ig

    IF Staining of U-251 using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68208-1-Ig

    IF Staining of HeLa using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of rat adrenal gland using 68208-1-Ig

    IHC staining of rat adrenal gland using 68208-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LETM1 antibody included
  • IHC staining of rat adrenal gland using 68208-1-Ig

    IHC staining of rat adrenal gland using 68208-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LETM1 antibody included
  • IF Staining of HeLa using 68208-1-Ig

    IF Staining of HeLa using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse adrenal gland using 68208-1-Ig

    IHC staining of mouse adrenal gland using 68208-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LETM1 antibody included
  • IHC staining of mouse adrenal gland using 68208-1-Ig

    IHC staining of mouse adrenal gland using 68208-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 68208-1-Ig (LETM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LETM1 antibody included
  • Featured Product
  • KD/KO验证

LETM1 Monoclonal antibody

LETM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68208-1-Ig

产品说明书

CloneNo. 2B9B9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Leucine zipper-EF-hand-containing transmembrane protein 1, 2B9B9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inrat adrenal gland tissue, mouse adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy LETM1 Ready-To-Use IHC Kit. LETM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68208-1-Ig targets LETM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 LETM1 fusion protein Ag8851 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine zipper-EF-hand containing transmembrane protein 1
别名 Leucine zipper-EF-hand-containing transmembrane protein 1, 2B9B9
计算分子量 739 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC021208
基因名称 LETM1
Gene ID (NCBI) 3954
RRIDAB_2935296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95202
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.

实验方案

Product Specific Protocols
WB protocol for LETM1 antibody 68208-1-IgDownload protocol
IHC protocol for LETM1 antibody 68208-1-IgDownload protocol
IF protocol for LETM1 antibody 68208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols