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SPOP Monoclonal antibody

SPOP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B9F2

Cat no : 68216-1-Ig

Print datasheet

Synonyms

HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2

验证数据展示

  • WB analysis using 68216-1-Ig

    WB analysis using 68216-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68216-1-Ig (SPOP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68216-1-Ig

    WB analysis using 68216-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68216-1-Ig (SPOP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, NCI-H1299 cells, U2OS cells, HCT-116 cells, HEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, MOLT-4 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68216-1-Ig targets SPOP in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SPOP fusion protein Ag10384 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name speckle-type POZ protein
Synonyms HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC003385
Gene Symbol SPOP
Gene ID (NCBI) 8405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43791
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SPOP (TEF2) protein was previously identified as an autoantigen in a patient with scleroderma pigmentosum. SPOP (speckle-type POZ protein), also known as TEF2, HIB homolog 1 or Roadkill homolog 1, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF Homology) domain and one C-terminal BTB/POZ domain. SPOP can exist as a homodimer and is expressed in a variety of tissues localizing to the nucleus. BTB-mediated SPOP dimers form linear oligomers via BACK domain dimerization, and we determine the concentration-dependent populations of the resulting oligomeric species (PMID: 27220849 ). Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. SPOP specifically interacts with CUL-3 via its BTB/POZ domain and recruits substrates to the CUL-3-based ubiquitin ligase via its MATH domain. Substrates recruited by SPOP and targeted for ubiquitylation via the CUL-3/SPOP complex include PDX-1, Bmi-1, MacroH2A, PIPK II ∫ and Daxx. These substrates are subsequently degraded by the proteasome. In addition, SPOP itself becomes ubiquitylated by the CUL-3-based ubiquitin ligase and is targeted for proteasomal degradation.

实验方案

Product Specific Protocols
WB protocol for SPOP antibody 68216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols