PDHB Monoclonal antibody
PDHB Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Rabbit, Pig
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A7C10
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse heart tissue |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68238-1-Ig targets PDHB in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
Tested Applications | IF/ICC, WB, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
Immunogen | PDHB fusion protein Ag6857 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | pyruvate dehydrogenase (lipoamide) beta |
Synonyms | DKFZp564K0164, PDHB, PDHE1 B, PHE1B |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC000439 |
Gene Symbol | PDHB |
Gene ID (NCBI) | 5162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P11177 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).
实验方案
Product Specific Protocols | |
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WB protocol for PDHB antibody 68238-1-Ig | Download protocol |
IF protocol for PDHB antibody 68238-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |