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BTN2A1 Monoclonal antibody

BTN2A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A6G2

Cat no : 68265-1-Ig

Print datasheet

Synonyms

BK14H9.1, BT2.1, BTF1, BTN2A1, DJ3E1.1, FLJ36567

验证数据展示

  • WB analysis using 68265-1-Ig

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68265-1-Ig

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68265-1-Ig targets BTN2A1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen BTN2A1 fusion protein Ag14533 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name butyrophilin, subfamily 2, member A1
Synonyms BK14H9.1, BT2.1, BTF1, BTN2A1, DJ3E1.1, FLJ36567
Calculated Molecular Weight 60 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC016661
Gene Symbol BTN2A1
Gene ID (NCBI) 11120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7KYR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)

实验方案

Product Specific Protocols
WB protocol for BTN2A1 antibody 68265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols