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PDXDC1 Monoclonal antibody

PDXDC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6E2

Cat no : 68278-1-Ig

Print datasheet

Synonyms

KIAA0251, LP8165, PDXDC1

验证数据展示

  • WB analysis using 68278-1-Ig

    WB analysis using 68278-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 68278-1-Ig

    IF Staining of MCF-7 using 68278-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 68278-1-Ig

    IF Staining of MCF-7 using 68278-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68278-1-Ig

    WB analysis using 68278-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells, pig colon tissue, rat colon tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68278-1-Ig targets PDXDC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen PDXDC1 fusion protein Ag15489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyridoxal-dependent decarboxylase domain containing 1
Synonyms KIAA0251, LP8165, PDXDC1
Calculated Molecular Weight 788 aa, 87 kDa
Observed Molecular Weight87 kDa
GenBank Accession NumberBC033748
Gene Symbol PDXDC1
Gene ID (NCBI) 23042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P996
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).

实验方案

Product Specific Protocols
WB protocol for PDXDC1 antibody 68278-1-IgDownload protocol
IF protocol for PDXDC1 antibody 68278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols