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MED23 Monoclonal antibody

MED23 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H4G4

Cat no : 68307-1-Ig

Print datasheet

Synonyms

ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DKFZp434H0117, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2

验证数据展示

  • WB analysis using 68307-1-Ig

    WB analysis using 68307-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68307-1-Ig (MED23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 68307-1-Ig

    WB analysis of 4T1 using 68307-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68307-1-Ig (MED23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68307-1-Ig targets MED23 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MED23 fusion protein Ag28483 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mediator complex subunit 23
Synonyms ARC130, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DKFZp434H0117, DRIP130, hSur 2, KIAA1216, MED23, mediator complex subunit 23, Protein sur 2 homolog, SUR2
Calculated Molecular Weight 156 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC060759
Gene Symbol MED23
Gene ID (NCBI) 9439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED23 antibody 68307-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols