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验证数据展示

  • WB analysis using 68310-1-Ig

    WB analysis using 68310-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68310-1-Ig (C6orf108 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HEK-293 using 68310-1-Ig

    IF Staining of HEK-293 using 68310-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C6orf108 antibody (68310-1-Ig, Clone: 2C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

C6orf108 Monoclonal antibody

C6orf108 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68310-1-Ig

产品说明书

CloneNo. 2C4E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DNPH1, EC:3.2.2.-, c-Myc-responsive protein RCL, c Myc responsive protein Rcl, 5-hydroxymethyl-dUMP N-hydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68310-1-Ig targets C6orf108 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C6orf108 fusion protein Ag22382 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 108
别名 DNPH1, EC:3.2.2.-, c-Myc-responsive protein RCL, c Myc responsive protein Rcl, 5-hydroxymethyl-dUMP N-hydrolase
计算分子量 174 aa, 19 kDa
观测分子量23 kDa
GenBank蛋白编号BC011683
基因名称 C6orf108
Gene ID (NCBI) 10591
RRIDAB_2935389
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43598
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).

实验方案

Product Specific Protocols
WB protocol for C6orf108 antibody 68310-1-IgDownload protocol
IF protocol for C6orf108 antibody 68310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols