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验证数据展示

  • WB analysis using 68327-1-Ig

    WB analysis using 68327-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat stomach using 68327-1-Ig

    WB analysis of rat stomach using 68327-1-Ig

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig small intestine using 68327-1-Ig

    WB analysis of pig small intestine using 68327-1-Ig

    pig small intestine tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68327-1-Ig

    IF Staining of A549 using 68327-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1B10 antibody (68327-1-Ig, Clone: 5A4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

AKR1B10 Monoclonal antibody

AKR1B10 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68327-1-Ig

产品说明书

CloneNo. 5A4G5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Pig, Rat

应用

WB, IF/ICC, ELISA

别名

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, rat stomach tissue, pig small intestine tissue, A549 cells, pig stomach tissue
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy AKR1B10 Ready-To-Use IHC Kit. AKR1B10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68327-1-Ig targets AKR1B10 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Pig, Rat
免疫原 AKR1B10 fusion protein Ag24406 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 aldo-keto reductase family 1, member B10 (aldose reductase)
别名 AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase
计算分子量 316 aa, 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC008837
基因名称 AKR1B10
Gene ID (NCBI) 57016
RRIDAB_2935399
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60218
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldo-keto reductase family 1 member B10 (AKR1B10), which belongs to the AKR superfamily, consists of 316 amino acids and its gene is located on chromosome 7q33. AKR1B10 was initially identified in liver cancer. It is highly expressed in the small intestine, colon, and adrenal gland.

实验方案

Product Specific Protocols
WB protocol for AKR1B10 antibody 68327-1-IgDownload protocol
IF protocol for AKR1B10 antibody 68327-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols