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GPKOW Monoclonal antibody

GPKOW Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat no : 68338-1-Ig

Print datasheet

Synonyms

G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54

验证数据展示

  • WB analysis using 68338-1-Ig

    WB analysis using 68338-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68338-1-Ig (GPKOW antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, LNcaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68338-1-Ig targets GPKOW in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GPKOW fusion protein Ag6545 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name G patch domain and KOW motifs
Synonyms G patch domain and KOW motifs, GPATC5, GPATCH5, GPKOW, Protein MOS2 homolog, Protein T54, T54
Calculated Molecular Weight 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC000397
Gene Symbol GPKOW
Gene ID (NCBI) 27238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92917
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPKOW is a RNA-binding protein involved in pre-mRNA splicing.It interacts directly with protein kinase A and protein kinase X and is also found associated with the spliceosome.

实验方案

Product Specific Protocols
WB protocol for GPKOW antibody 68338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols