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SGF29 Monoclonal antibody

SGF29 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1B4

Cat no : 68339-1-Ig

Print datasheet

Synonyms

CCDC101, FLJ32446, SGF29

验证数据展示

  • WB analysis using 68339-1-Ig

    WB analysis using 68339-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68339-1-Ig (CCDC101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68339-1-Ig targets SGF29 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen SGF29 fusion protein Ag33219 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil domain containing 101
Synonyms CCDC101, FLJ32446, SGF29
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011981
Gene Symbol SGF29
Gene ID (NCBI) 112869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96ES7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

实验方案

Product Specific Protocols
WB protocol for SGF29 antibody 68339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols