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验证数据展示

  • WB analysis using 68360-1-Ig

    WB analysis using 68360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68360-1-Ig (MCTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68360-1-Ig

    IF Staining of HeLa using 68360-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MCTS1 antibody (68360-1-Ig, Clone: 1F10C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

MCTS1 Monoclonal antibody

MCTS1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68360-1-Ig

产品说明书

CloneNo. 1F10C7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 1F10C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, Raji cells, Jurkat cells, K-562 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68360-1-Ig targets MCTS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MCTS1 fusion protein Ag7057 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 malignant T cell amplified sequence 1
别名 Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 1F10C7
计算分子量 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC001013
基因名称 MCTS1
Gene ID (NCBI) 28985
RRIDAB_3085082
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULC4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

实验方案

Product Specific Protocols
WB protocol for MCTS1 antibody 68360-1-IgDownload protocol
IF protocol for MCTS1 antibody 68360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols