验证数据展示
经过测试的应用
| Positive WB detected in | OS-RC-2 cells, HuH-7 cells, human placenta tissue, HUVEC cells, pig kidney tissue, rabbit kidney tissue, Caki-2 cells, Karpas-299 cells |
| Positive IF/ICC detected in | HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
68383-1-Ig targets DPP4/CD26 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, pig, rabbit |
| 文献引用反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dipeptidyl-peptidase 4 |
| 别名 | CD26, ADCP-2, ADCP2, ADCP 2, ADABP |
| 计算分子量 | 88 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC013329 |
| 基因名称 | DPP4 |
| Gene ID (NCBI) | 1803 |
| RRID | AB_3085101 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P27487 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DPP4/CD26 antibody 68383-1-Ig | Download protocol |
| IF protocol for DPP4/CD26 antibody 68383-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
