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ANKRD54 Monoclonal antibody

ANKRD54 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A9

Cat no : 68388-1-Ig

Print datasheet

Synonyms

ANKRD54, ankyrin repeat domain 54

验证数据展示

  • WB analysis using 68388-1-Ig

    WB analysis using 68388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of U2OS using 68388-1-Ig

    WB analysis of U2OS using 68388-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68388-1-Ig targets ANKRD54 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ANKRD54 fusion protein Ag22772 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ankyrin repeat domain 54
Synonyms ANKRD54, ankyrin repeat domain 54
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NXT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.

实验方案

Product Specific Protocols
WB protocol for ANKRD54 antibody 68388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols