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ERGIC3 Monoclonal antibody

ERGIC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9F11

Cat no : 68398-1-Ig

Print datasheet

Synonyms

C20orf47, CGI 54, dJ477O4.2, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

验证数据展示

  • WB analysis using 68398-1-Ig

    WB analysis using 68398-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNCI-H1299 cells, Calu-3 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68398-1-Ig targets ERGIC3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ERGIC3 fusion protein Ag9190 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ERGIC and golgi 3
Synonyms C20orf47, CGI 54, dJ477O4.2, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009765
Gene Symbol ERGIC3
Gene ID (NCBI) 51614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y282
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)

实验方案

Product Specific Protocols
WB protocol for ERGIC3 antibody 68398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols