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验证数据展示

  • WB analysis using 68398-1-Ig

    WB analysis using 68398-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68398-1-Ig

    WB analysis of HepG2 using 68398-1-Ig

    WB result of ERGIC3 antibody (68398-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC3 transfected HepG2 cells.

ERGIC3 Monoclonal antibody

ERGIC3 Monoclonal Antibody for WB, ELISA
Cat No. 68398-1-Ig

产品说明书

CloneNo. 1B9F11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54, C20orf47

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, HepG2 cells, Calu-3 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68398-1-Ig targets ERGIC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERGIC3 fusion protein Ag9190 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ERGIC and golgi 3
别名 ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54, C20orf47
计算分子量 383 aa, 43 kDa
观测分子量 50 kDa
GenBank蛋白编号BC009765
基因名称 ERGIC3
Gene ID (NCBI) 51614
RRIDAB_3085115
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y282
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)

实验方案

Product Specific Protocols
WB protocol for ERGIC3 antibody 68398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols