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HIP1R Monoclonal antibody

HIP1R Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F10D10

Cat no : 68403-1-Ig

Print datasheet

Synonyms

FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

验证数据展示

  • WB analysis using 68403-1-Ig

    WB analysis using 68403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 68403-1-Ig

    WB analysis of 4T1 using 68403-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68403-1-Ig

    IF Staining of HeLa using 68403-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HIP1R antibody (68403-1-Ig, Clone: 2F10D10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA549 cells, 4T1 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68403-1-Ig targets HIP1R in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HIP1R fusion protein Ag10657 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name huntingtin interacting protein 1 related
Synonyms FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655
Calculated Molecular Weight 615 aa, 70 kDa, 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC067085
Gene Symbol HIP1R
Gene ID (NCBI) 9026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75146
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIP1R antibody 68403-1-IgDownload protocol
IF protocol for HIP1R antibody 68403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols