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验证数据展示

  • WB analysis using 68422-1-Ig

    WB analysis using 68422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68422-1-Ig (UBOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68422-1-Ig

    WB analysis using 68422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68422-1-Ig (UBOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

UBOX5 Monoclonal antibody

UBOX5 Monoclonal Antibody for WB, ELISA
Cat No. 68422-1-Ig

产品说明书

CloneNo. 2A7A4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

KIAA0860, RING finger protein 37, RNF37, U box domain containing 5, UBCE7IP5, UBOX5, UIP5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Saos-2 cells, T-47D cells, SK-BR-3 cells, A549 cells, K-562 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68422-1-Ig targets UBOX5 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag6573

Product name: Recombinant human UBOX5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 196-541 aa of BC000515

Sequence: QPAKTCSQEVIDSILLVTSENLPQDVALQAPALPMESDCDPGDQPESQQAPSSLQKLAEIIQDVPEEFLDPITLEIMPCPMLLPSGKVIDQSTLEKCNRSEATWGRVPSDPFTGVAFTPHSQPLPHPSLKARIDHFLLQHSIPGCHLLGRAQTALAVIPSSIVLPSQKRKIEQAEHVPDSNFGVNASCFSATSPLVLPTTSEHTAKKMKATNEPSLTHMDCSTGPLSHEQKLSQSLEIALASTLGSMPSFTARLTRGQLQHLGTRGSNTSWRPGTGSEQPGSILGPECASCKRVFSPYFKKEPVYQLPCGHLLCRPCLGEKQRSLPMTCTACQRPVASQDVLRVHF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 U-box domain containing 5
别名 KIAA0860, RING finger protein 37, RNF37, U box domain containing 5, UBCE7IP5, UBOX5, UIP5
计算分子量 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC000515
基因名称 UBOX5
Gene ID (NCBI) 22888
RRIDAB_3085137
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBOX5 antibody 68422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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