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验证数据展示

  • WB analysis using 68430-1-Ig

    WB analysis using 68430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68430-1-Ig (Synaptotagmin-9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Synaptotagmin-9 Monoclonal antibody

Synaptotagmin-9 Monoclonal Antibody for WB, ELISA
Cat No. 68430-1-Ig

产品说明书

CloneNo. 3D2F2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, ELISA

别名

SytIX, SYT9, Synaptotagmin IX, 3D2F2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrabbit retina tissue, rat retina tissue, mouse retina tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68430-1-Ig targets Synaptotagmin-9 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 Synaptotagmin-9 fusion protein Ag33194 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptotagmin IX
别名 SytIX, SYT9, Synaptotagmin IX, 3D2F2
计算分子量 491 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC029605
基因名称 Synaptotagmin-9
Gene ID (NCBI) 143425
RRIDAB_3085144
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86SS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 68430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols