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CAMK4 Monoclonal antibody

CAMK4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7A7

Cat no : 68452-1-Ig

Print datasheet

Synonyms

CaM kinase GR, CaMK GR, CaMK IV, CAMK4

验证数据展示

  • WB analysis using 68452-1-Ig

    WB analysis using 68452-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68452-1-Ig (CAMK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue, rat thymus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68452-1-Ig targets CAMK4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CAMK4 fusion protein Ag33332 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase IV
Synonyms CaM kinase GR, CaMK GR, CaMK IV, CAMK4
Calculated Molecular Weight 473 aa, 52 kDa
Observed Molecular Weight56-65 kDa
GenBank Accession NumberBC025687
Gene Symbol CAMK4
Gene ID (NCBI) 814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16566
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)

实验方案

Product Specific Protocols
WB protocol for CAMK4 antibody 68452-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols