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验证数据展示

  • WB analysis using 68456-1-Ig

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68456-1-Ig

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68456-1-Ig

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

EXOSC7 Monoclonal antibody

EXOSC7 Monoclonal Antibody for WB, ELISA
Cat No. 68456-1-Ig

产品说明书

CloneNo. 2A4C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42, EAP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSaos-2 cells, HSC-T6 cells, PANC-1 cells, NIH/3T3 cells, 4TA cells, HT-29 cells, LNCaP cells, MDA-MB-231 cells, IMR-32 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, pig brain tissue, Caco-2 cells, HeLa cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC7 Ready-To-Use IHC Kit. EXOSC7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68456-1-Ig targets EXOSC7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 EXOSC7 fusion protein Ag18322 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 7
别名 KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42, EAP1
计算分子量 291 aa, 32 kDa
观测分子量35 kDa
GenBank蛋白编号BC012831
基因名称 EXOSC7
Gene ID (NCBI) 23016
RRIDAB_3085168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15024
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA.

实验方案

Product Specific Protocols
WB protocol for EXOSC7 antibody 68456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols