验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68464-1-Ig targets TEAD3 in WB, ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 | TEAD3 fusion protein Ag4407 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TEA domain family member 3 |
| 别名 | DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5 |
| 计算分子量 | 435 aa, 49 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC027877 |
| 基因名称 | TEAD3 |
| Gene ID (NCBI) | 7005 |
| RRID | AB_3085175 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99594 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TEAD3 antibody 68464-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |