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STEAP4 Monoclonal antibody

STEAP4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12G6

Cat no : 68465-1-Ig

Print datasheet

Synonyms

DKFZp666D049, FLJ23153, Metalloreductase STEAP4, STAMP2, STEAP family member 4, STEAP4, TIARP, TNFAIP9

验证数据展示

  • WB analysis using 68465-1-Ig

    WB analysis using 68465-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inA549 cells, HaCaT cells, HeLa cells, MDA-MB-468 cells, SK-BR-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68465-1-Ig targets STEAP4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen STEAP4 fusion protein Ag30137 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name STEAP family member 4
Synonyms DKFZp666D049, FLJ23153, Metalloreductase STEAP4, STAMP2, STEAP family member 4, STEAP4, TIARP, TNFAIP9
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC020600
Gene Symbol STEAP4
Gene ID (NCBI) 79689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ687X5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

实验方案

Product Specific Protocols
WB protocol for STEAP4 antibody 68465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols