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验证数据展示

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

RHOT2 Monoclonal antibody

RHOT2 Monoclonal Antibody for WB, ELISA
Cat No. 68469-1-Ig

产品说明书

CloneNo. 1A3C2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

ARHT2, C16orf39, hMiro 2, MIRO 2, Mitochondrial Rho GTPase 2, RASL, RHOT2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68469-1-Ig targets RHOT2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 RHOT2 fusion protein Ag29690 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member T2
别名 ARHT2, C16orf39, hMiro 2, MIRO 2, Mitochondrial Rho GTPase 2, RASL, RHOT2
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC014942
基因名称 RHOT2
Gene ID (NCBI) 89941
RRIDAB_3085180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IXI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOT2 (Mitochondrial Rho GTPase 2) is also named as MIRO-2, hMiro-2, ARHT2, C16orf39 and Ras homolog gene family member T2. RHOT2 belongs to the mitochondrial Rho GTPase family. RHOT2 gene encodes a member of Rho family of GTPase, which are localized to the outer membrane of mitochondria (PMID: 22078885).

实验方案

Product Specific Protocols
WB protocol for RHOT2 antibody 68469-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols