• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AMY2B Monoclonal antibody

AMY2B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E5G2

Cat no : 68474-1-Ig

Print datasheet

Synonyms

Alpha amylase 2B, AMY2, AMY2B, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase

验证数据展示

  • WB analysis using 68474-1-Ig

    WB analysis using 68474-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68474-1-Ig (AMY2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig pancreas tissue, rat pancreas tissue，mouse pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68474-1-Ig targets AMY2B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen AMY2B fusion protein Ag3260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amylase, alpha 2B (pancreatic)
Synonyms Alpha amylase 2B, AMY2, AMY2B, amylase, alpha 2B (pancreatic), Carcinoid alpha amylase
Calculated Molecular Weight 511 aa, 58 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC011179
Gene Symbol AMY2B
Gene ID (NCBI) 280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012).

实验方案

Product Specific Protocols
WB protocol for AMY2B antibody 68474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols