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ATP1A1/2 Monoclonal antibody

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8G1

Cat no : 68505-1-Ig

Print datasheet

Synonyms

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

验证数据展示

  • WB analysis using 68505-1-Ig

    WB analysis using 68505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of SK-BR-3 using 68505-1-Ig

    WB analysis of SK-BR-3 using 68505-1-Ig

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 68505-1-Ig

    WB analysis of rat kidney using 68505-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 68505-1-Ig

    WB analysis of pig brain using 68505-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 68505-1-Ig

    IF Staining of C2C12 using 68505-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inrat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue
Positive IF/ICC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68505-1-Ig targets ATP1A1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ATP1A1/2 fusion protein Ag10489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Synonyms ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase
Calculated Molecular Weight 1020 aa, 112 kDa
Observed Molecular Weight 97-100 kDa
GenBank Accession NumberBC052271
Gene Symbol ATP1A2
Gene ID (NCBI) 477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50993
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.

实验方案

Product Specific Protocols
WB protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
IF protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols