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UBR2 Monoclonal antibody

UBR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D5H2

Cat no : 68565-1-Ig

Print datasheet

Synonyms

bA49A4.1, C6orf133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, KIAA0349, N recognin 2, RP3 392M17.3, UBR2

验证数据展示

  • WB analysis using 68565-1-Ig

    WB analysis using 68565-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68565-1-Ig (UBR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68565-1-Ig targets UBR2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen UBR2 fusion protein Ag13446 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin protein ligase E3 component n-recognin 2
Synonyms bA49A4.1, C6orf133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, KIAA0349, N recognin 2, RP3 392M17.3, UBR2
Calculated Molecular Weight439aa,50 kDa; 1755aa,201 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC064512
Gene Symbol UBR2
Gene ID (NCBI) 23304
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IWV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for UBR2 antibody 68565-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols