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验证数据展示

  • WB analysis using 68569-1-Ig

    WB analysis using 68569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68569-1-Ig

    WB analysis using 68569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

SETD7 Monoclonal antibody

SETD7 Monoclonal Antibody for WB, ELISA
Cat No. 68569-1-Ig

产品说明书

CloneNo. 1C1A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, MCF-7 cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68569-1-Ig targets SETD7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SETD7 fusion protein Ag33803 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET domain containing (lysine methyltransferase) 7
别名 FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7
计算分子量 366 aa, 41 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC121055
基因名称 SETD7
Gene ID (NCBI) 80854
RRIDAB_3085271
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WTS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

实验方案

Product Specific Protocols
WB protocol for SETD7 antibody 68569-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols