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IQGAP1 Monoclonal antibody

IQGAP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E11A2

Cat no : 68588-1-Ig

Print datasheet

Synonyms

Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2

验证数据展示

  • WB analysis using 68588-1-Ig

    WB analysis using 68588-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68588-1-Ig (IQGAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 68588-1-Ig

    IHC staining of human skin cancer using 68588-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68588-1-Ig (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included

经过测试的应用

Positive WB detected inA431 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68588-1-Ig targets IQGAP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen IQGAP1 fusion protein Ag19321 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IQ motif containing GTPase activating protein 1
Synonyms Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2
Calculated Molecular Weight 189 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC151834
Gene Symbol IQGAP1
Gene ID (NCBI) 8826
ENSEMBL Gene IDENSG00000140575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46940
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

实验方案

Product Specific Protocols
WB protocol for IQGAP1 antibody 68588-1-IgDownload protocol
IHC protocol for IQGAP1 antibody 68588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols